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题名: Colorimetric biosensing of mercury(II) ion using unmodified gold nanoparticle probes and thrombin-binding aptamer
作者: Wang Y ; Yang F ; Yang XR
刊名: BIOSENSORS & BIOELECTRONICS
出版日期: 2010
卷号: 25, 期号:8, 页码:1994-1998
关键词: SPECTROPHOTOMETRIC DETERMINATION ; ATOMIC-ABSORPTION ; BASE-PAIRS ; HG-II ; DNA ; HYBRIDS ; SENSOR
通讯作者: Yang XR
中文摘要: A colorimetric assay for the determination of mercury(II) (Hg2+) in the presence of lead(II) (Pb2+) was demonstrated with unmodified gold nanoparticles (AuNPs) as probes and 15-met thrombin-binding aptamer (TBA, 5'-GGTTGGTGTGGTTGG-3') as sensing elements. Upon the addition of Hg2+ or Pb2+, TBA consisting of six thymidine units and nine guanosine units interacted specifically with both ions to form a hairpin-like or a quadruplex structure, respectively. As a result, these conformation changes facilitated the salt-induced AuNP aggregation. Subsequently, to eliminate Pb2+ interference in the determination of Hg2+, a novel technique by the use of a characteristic wavelength of aggregated AuNPs instead of the universal masking agent of Pb2+ (2,6-pyridinedicarboxylic acid, PDCA) was herein proposed. A comparison of the absorption spectra of the aggregated AuNPs in the presence of Hg2+ and Pb2+ showed that the characteristic wavelength of the aggregated AuNPs (800 nm) facilitated the determination of Hg2+ in the presence of Pb2+. The calibration curve showed that the absorbance value at 800 nm increased linearly over the Hg2+ concentration range of 0.39-8.89 mu M with a limit of detection of 200 nM. Then, the assay was successfully employed to determine Hg2+ in several water samples. (c) 2010 Elsevier B.V. All rights reserved.
收录类别: SCI收录期刊论文
语种: 英语
WOS记录号: WOS:000276424200022
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内容类型: 期刊论文
URI标识: http://ir.ciac.jl.cn/handle/322003/43808
Appears in Collections:长春应用化学研究所知识产出_期刊论文

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Wang Y;Yang F;Yang XR.Colorimetric biosensing of mercury(II) ion using unmodified gold nanoparticle probes and thrombin-binding aptamer,BIOSENSORS & BIOELECTRONICS,2010,25(8):1994-1998
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